That has left interim gaffers Andy Whiteford and Davie Wilson with the daunting task of taking the team to face Premier League outfit Pollok in the Scottish Junior Cup this afternoon.
Fox handed in his resignation following the 3-2 defeat at the hands of Shettleson last week.
That result left the Farmer’s Boys 10th in the league, and 11 points off the pace after eight games played.
Now coaches Whiteford and Wilson, who both agreed to fill the hotseat in the short term, face the challenge of assembling a side to travel to Newlandsfield to play a ‘Lok side who have already put them out of two competitions this season.
Whiteford said: “I spoke to Robert on Tuesday and he told me he had spent a few days thinking about it, and felt it was the right time to resign from his position.
“Everyone had been disappointed with the result on Saturday, including Robert, but I don’t think that was the main reason for him going.
“Until I speak to him at length, I won’t know all the factors which led to him making that decision.”
Pollok have been responsible for knocking Neilston out of both the League Cup and West Cup so far this season, scoring seven without reply over the two matches.
But Whiteford insists the Emirates Scottish Junior Cup tie will make his job easier today, not harder.
He added: “There’s no doubt the Pollok game is going to be tough, and not a lot of people will give us much of a chance.
“However, from our point of view, it’s a great game for the players to focus on.
“These are the games players want to play in and, if they can’t get themselves up for a Scottish cup match at Newlandsfield, then something is wrong.
“As far as I’m concerned, I’ll just need to pick a team, and let the players get out there.”
Fox, who spent a year at Neilston as a coach before serving a similar stint as club manager, was unavailable for comment following his midweek resignation.